Quick Answer: What Is The Purpose Of Biochemical Testing?

What are the criteria for classification of bacteria?

Indeed, bacteria are classified on the basis of many characteristics.

Cell shape, nature of multicell aggregates, motility, formation of spores, and reaction to the Gram stain are important..

What is biochemical diagnosis?

Biochemistry combines analytical chemistry with aspects of physiology, physical chemistry, pathophysiology and diagnostic medicine. Diagnostic Clinical Biochemistry uses biochemical knowledge and techniques to assist in the diagnosis of human disease, to follow its progress and to monitor the effect of treatment.

What is the principle of biochemical test?

Principle: This test determines the capability of some organisms to produce non-acidic or neutralend products, such as acetyl methyl corbinol (acetoin), from the organic acid that results fromglucose metabolism.

What are the four major types of biochemical reactions?

Identify the four main types of biochemical reactions (oxidation-reduction, hydrolysis, condensation, and neutralization). Describe the role of enzymes in biochemical reactions (related to activation energy).

What do you mean by biochemical reaction?

A biochemical reaction is the transformation of one molecule to a different molecule inside a cell. Biochemical reactions are mediated by enzymes, which are biological catalysts that can alter the rate and specificity of chemical reactions inside cells.

What are the methods used to identify bacteria?

Among the techniques we use are: DNA sequencing – to identify bacteria, moulds and yeasts. Riboprinter analysis – for bacterial identification and characterisation. Repeat–based polymerase chain reaction – for assessing the similarity of microorganisms.

What are cultural characteristics of bacteria?

Some bacteria express certain characteristics, such as the formation of pigment, more strongly at some temperatures than at others. The length of time for which the bacteria is incubated may also affect colonial characteristics such as colony size. The presence of other organisms can interfere with bacterial growth.

How are biochemical tests used to identify bacteria?

Tests used to identify Gram Positive BacteriaCatalase Test.Mannitol Salt Agar (MSA)Blood Agar Plates (BAP) Streak-stab technique.Taxos P (optochin sensitivity testing)Taxos A (bacitracin sensitivity testing)CAMP Test.Bile Esculin Agar.Nitrate Broth.More items…

What are biochemical characteristics?

The biochemical characteristics of a bacterium provide many traits that are useful for classification and identification. Analyzing the nutritional and metabolic capabilities of the bacterial isolate is a common approach for determining the genus and the species of the bacterium.

What is a catalase test used for?

The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme, catalase, is produced by bacteria that respire using oxygen, and protects them from the toxic by-products of oxygen metabolism.

What is the IMViC test?

The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. … The term “IMViC” is an acronym for each of these tests. “I” is for indole test; “M” is for methyl red test; “V” is for Voges-Proskauer test, and “C” is for citrate test.

What are biochemical blood tests?

The biochemical profile is a series of blood tests used to evaluate the functional capacity of several critical organs and systems, such as the liver and kidneys. These tests can be done on an empty stomach or not, and are usually accompanied by a complete blood count (CBC).

What are biochemical tests used for?

Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one type of organism to another.

What is an example of a biochemical reaction?

Biochemical reactions are chemical reactions within living things. For example, photosynthesis. How do enzymes speed up biochemical reactions? They reduce the amount to necessary activation energy.

Why is it important to be able to identify the types of bacteria in a clinical sample?

In microbial ecology, the identification of microorganisms helps us characterize biodiversity. … Because the clinical samples will most likely contain many microorganisms, both normal flora and pathogens, it is important to isolate the pathogen in a pure culture using various types of selective and differential media.

How do you prepare for a biochemical test?

Test ProcedureDilute your organism in a tube of sterile water to obtain a turbidity equivalent to the 0.5 McFarland test standard. … Using a sterile 1mL pipette, place 1 mL of organism into the middle of the tube.Cap tightly; do not jostle.Incubate for 24 hours at 37°C.

Why are biochemical tests important?

Biochemical tests are among the most important methods for microbial identification. … Microbial biochemistry tests shorten the time required to identify microbes, reduce costs, and ensure or enhance the accuracy of identification of an unknown sample. It is the fastest developing trend in microbial identification.

What is a biochemical process?

Biochemical processes are the chemical processes that occur in living organisms, involving biomolecules. … One of the first biochemical processes studied was alcoholic fermentation: the conversion of sugars into cellular energy with ethanol (alcohol) and carbon dioxide as by-products.